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Tuesday, August 4, 2020 | History

2 edition of Studies on protein antigens of mycobacteria found in the catalog.

Studies on protein antigens of mycobacteria

Effiong Okon Etim Bassey

Studies on protein antigens of mycobacteria

a comparative study of "mycobacterium tuberculosis" and "mycobacterium bovis"

by Effiong Okon Etim Bassey

  • 179 Want to read
  • 33 Currently reading

Published by University of Birmingham in Birmingham .
Written in English


Edition Notes

Thesis (Ph.D)-University of Birmingham, Dept of Immunology, 1991.

Statementby Effiong Okon Etim Bassey.
ID Numbers
Open LibraryOL13929204M

  The goal is to find the set of antigens, in TB patients’ hemolysate, which is related to Mycobacterium tuberculosis bacilli. The approach taken follows the following steps Figure 6: The study resources are: [A] Patients [B] Mycobacterium tuberculosis (H37Rv) For each patient: Collect blood sample on anticoagulant (step 1). Substances elaborated by bacteria that have antigenic activity. | Explore the latest full-text research PDFs, articles, conference papers, preprints and more on BACTERIAL ANTIGENS. Find methods.

Monoclonal antibodies directed against M. leprae specific antigens 3–7 have been used to isolate the genes encoding the five most immunogenic protein antigens of the leprosy bacillus.   This study showed that Agrobacterium-mediated transient transformation of maize seedlings allows the production of the Ag85B antigen from Mycobacterium avium subsp. paratuberculosis (MAP) at levels up to µg g −1 dry weight. Moreover, BALB/c mice orally and subcutaneously immunized with the maize-made Ag85B vaccine produced specific IgG.

In this study, in vitro selections against the latency associated Mycobacterium tuberculosis (Mtb) heat shock protein 16 kDa antigen (16 kDa) presented by HLA-A* and HLA-A*24 were carried out with the use of a human domain phage antibody library.   The glycoprotein APA (Alanine- and Proline-rich Antigen, a 45/47 kDa antigen complex, Rv) is considered as a major immunodominant antigen secreted by M. tuberculosis. This antigen has proved to be highly immunogenic in experimental models and humans, presenting a significant potential for further development of a new vaccine for tuberculosis. Glycosylation plays a .


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Studies on protein antigens of mycobacteria by Effiong Okon Etim Bassey Download PDF EPUB FB2

Differential delayed-type hypersensitivity skin testing with tuberculin purified protein derivatives from Mycobacterium bovis and M.

avium is the standard for Studies on protein antigens of mycobacteria book bovine tuberculosis. However, improved tests based on defined, specific antigens are urgently needed. In the present study, a combination of bioinformatics, molecular biology, and bovine models of infection were used to Cited by: Proteins released by Mycobacterium tuberculosis to the extracellular environment have been the focus of much of the research directed at identifying antigens that induce protective immunity or those that elicit immune responses of diagnostic value (reviewed in references 2, 11, and 37).Two different experimental approaches have been used.

One is analysis of the protein composition of M Cited by: The presence of mycobacterial antigens in leprosy skin lesions was studied by immunohistological methods using monoclonal antibodies (MAbs) to Mycobacterium leprae-specific phenolic glycolipid I (PGL-I) and to cross-reactive mycobacterial antigens of 36 kd, 65 kd, and lipoarabinomannan (LAM).The staining patterns with MAb to 36 kd and 65 kd were heterogeneous and were also seen in the lesions Cited by: S.H.

Gillespie MB, BCh, BAO, MRCP(UK), MRCPath, in Medical Microbiology Illustrated, Immunological identification. Mycobacterial antigens are divided into four groups (i–iv): group i antigens are shared with all mycobacteria, nocardia and some corynebacteria; group ii antigens are found in slow-growing mycobacteria; group iii antigens occur in rapid-growing organisms; and group iv.

Isoe1ectric focusing showed that the proteins of all mycobacteria focused within the pH range of toexcept those of M. leprae which extended beyond to These studies have indicated for the first time that the protein antigens of mycobacteria are acidic in by: 2.

Presently, purified protein derivative (PPD) harvested from liquid culture of MAP is used as antigenic marker of DTH assay. However, specificity of these assays is under scrutiny due to presence of similar antigens in other mycobacteria (Facciuolo et al., ).

Therefore, an alternate DTH test based on MAP specific antigens active during the. The aim of our work here was to evaluate the immunogenicity of 60 mycobacterial antigens, some of which have not been previously assessed, notably a novel series of in vivo-expressed Mycobacterium tuberculosis (IVE-TB) antigens.

We enrolled subjects and separated them in individuals with and without latent tuberculosis infection (LTBI) vs. patients with active tuberculosis (TB). Mpt64 is one antigen capable of eliciting a distinctive response in people with active TB. A Mptbased TB patch test developed by Sequella has been shown repeatedly to yield negative results in patients with prior BCG vaccination, those who are infected with other Mycobacteria, and those who have been cured of TB.

48 In studies carried out in Japan and the Philippines, the Mpt64 skin patch. Mycobacterium bovis bacillus Calmette-Guérin (BCG) vaccination is efficacious for newborns or adults with no previous exposure to environmental mycobacteria. To determine the relative contribution and the nature of γδ T-cell receptor-positive T cells in newborns, compared to CD4 + T cells, in immunity induced by M.

bovis BCG vaccination, 4-week-old specific-pathogen-free pigs were. Mycobacteria are the unique group of bacteria that are currently used in antitumoral immunotherapy. Specifically, intravesical instillation of viable cells of Mycobacterium bovis Bacillus Calmette-Guérin (BCG), after transurethral resection of non-muscle invasive bladder cancer, is the most efficacious treatment for avoiding recurrence and progression of the disease.

Well now, immunoglobulins participating in protection against Mtb have affinity for different mycobacterial antigens on cell envelop (i.e. arabinomannan, lipoarabinomannan, lipoglypoproteins and polysaccharides) and just a few immunogenic proteins, like heparin binding haemagglutinin (HBHA) and the Mtb 16 kDa protein (HspX).

The mycobacteria's. These antigens (12, 19, 22a, b, 24, 25, 30, 32, 39, 65 and 70 kDa) included the majority of M.

bovis protein antigens described to date and are highly homologous to those purified from M. Demonstration of soluble antigens in leprosy nodules by immunodiffusion.

Int J Lepr Other Mycobact Dis. Apr-Jun; 38 (2)– Abe M, Minagawa F, Yoshino Y, Okamura K. Studies on the antigenic specificity of Mycobacterium leprae. Purification and immunological characterization of the soluble antigen in leprosy by:   Detection of Mycobacterium avium subspecies paratuberculosis (MAP) infection is key to the control of Johne’s disease.

Immunohistochemistry is one of the methods of detection of MAP infection in tissues. However, unavailability of commercial antibodies that can detect the organism is a limiting factor for the use of immunohistochemistry.

This study was aimed at developing an. The identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. This study was designed for cloning and expression of ESAT-6 as a potent antigen of Mycobacterium tuberculosis.

Selected gene (Rv) was amplified by PCR and product was ligated into expressing plasmid vector pQE30 and recombinant pQEES.

A critical review of the current and most recent advances in the genomics and molecular biology of mycobacteria. Focuses on the topical and most relevant aspects. Includes strain variation and evolution, hypervirulent strains, electron transport and respiration, lipid biosynthesis, DNA repair, oxygen signaling, sulphur metabolism, protein secretion, the protein kinase family.

The immunostimulant Imiquimod, a Toll-like receptor-7 (TLR-7) agonist, was encapsulated in the oily core and a fusion protein, formed by two antigens of Mtb, was absorbed either onto the NC surface (CS:Ag and INU:pArg:Ag) or between two polymer layers (INU:Ag:pArg) in order to assess the influence of the antigen positioning on the immune response.

Abstract. A study on skin cross-reactivity between stabilized 14 C-labeled mycobacterial antigens, namely tuberculin purified protein derivative (PPD; from Mycobacterium tuberculosis), PPD-A (M. avium), PPD-Y (M. kansasii), PPD-G (M. scrofulaceum), PPD-B (M.

intracellulare), and PPD-F (M. fortuitum), has been carried out in groups of guinea pigs sensitized with one of the following heat-killed. During persistent infection and hypoxic-stress, Mycobacterium tuberculosis (Mtb) expresses a series of Mtb latency antigens.

The aim of this study was to evaluate the immunogenicity of a DNA vaccine encoding the Mtb latency antigen Rvc and to explore the effect of pulmonary delivery and co-formulation with poly (d,l-lactide-co-glycolide) (PLGA)-polyethyleneimine (PEI) nanoparticles (np) on.

The aim of the present study was cloning, expression and purification of MPT as a protein antigen of M. bovis in a prokaryotic system for the usage in the future diagnostic studies. Mycobacterium Tuberculosis HSP is a major antigen maximally expressed during the stationary phase.

Previous studies showed that HSP can function as a molecular chaperone in vitro. Here, crystallization trails of HSP were reported. Huang, H., Wang, C., Rubelt, F.

et al. Analyzing the Mycobacterium tuberculosis immune response by T-cell receptor clustering with GLIPH2 and genome-wide antigen screening.

Nat Biotechnol (Branded Books; About. About Clinical Infectious Diseases; Sadamu Nagai, Protein Antigens of Mycobacteria Studied by Quantitative Immunologic Techniques, Clinical Infectious Diseases, Vol Issue 1, JanuaryPages –, single ascending dose study and a volunteer infection study to characterize the safety.